Nazli Ayhan1, Amal Baklouti1, Jorian Prudhomme2, Gernot Walder3, Fatima Amaro4, Bulent Alten5, Sara Moutailler6, Koray Ergunay7, Remi N Charrel1, Hartwig Huemer8
Vector Borne Zoonotic Dis. 2017 Jan;17(1):73-80. doi: 10.1089/vbz.2016.1957.
The purpose of this review is to provide practical information to help researchers intending to perform „from field to laboratory“ studies on phleboviruses transmitted by sandflies. This guideline addresses the different steps to be considered starting from the field collection of sandflies to the laboratory techniques aiming at the detection, isolation, and characterization of sandfly-borne phleboviruses. In this guideline article, we address the impact of various types of data for an optimal organization of the field work intending to collect wildlife sandflies for subsequent virology studies. Analysis of different data sets should result in the geographic positioning of the trapping stations. The overall planning, the equipment and tools needed, the manpower to be deployed, and the logistics to be anticipated and set up should be organized according to the objectives of the field study for optimal efficiency.
Keywords: Bunyaviridae; Toscana virus; arbovirus(es); field studies; sand fly (flies); vector-borne.
Links: PMID: 28055576; DOI: 10.1089/vbz.2016.1957